Table 1.

Primers and temperatures of annealing adopted for RT-PCR experiments.

Gene	Forward	Reverse	T (°C)	Amplicon size (bp)
Tumor necrosis factor alpha (Tnfa)	5′-CCCTCACACTCAGATCATCTTCT-3′	5′-GCTACGACGTGGGCTACAG-3′	55°C	61
Interferon beta 1 (Ifnb)	5′-CAGCTCCAAGAAAGGACGAAC-3′	5′-GGCAGTGTAACTCTTCTGCAT-3′	56°C	138
Interferon-induced protein with tetratricopeptide repeats 2 (Ifit2)	5′-AGAACCAAAACGAGAGAGTGAAG-3′	5′-TCCAGACGGTAGTTCGCAATG-3′	57°C	106
Glyceraldehyde 3-phosphate dehydrogenase (Gapdh)	5′-TGT TCC TAC CCC CAA TGT GT-3′	5′-GGT CCT CAG TGT AGC CCA AG-3′	57°C	137