Figure 6.

miR-155 induced CD19⁺CD24^hiCD27⁺ B cells to produce higher levels of TNFα in Crohn’s disease (CD) patients. (A) CD19⁺CD24^hiCD27⁺ B cells from healthy controls (HCs) and CD patients (N = 15) were sorted and stimulated with 100 nM CpG oligonucleotides for 24 h; then, miR-155 expression was determined by Q-PCR. (B–E) CD19⁺CD24^hiCD27⁺ B cells from HCs and CD patients (N = 3) were sorted and electroporated with miR-155 mimic, inhibitor or control and cultured for another 48 h under stimulation with 100 nM CpG oligonucleotides. (B) Then, FACS was used to measure TNFα and interleukin-10 (IL-10) expression. The percentages of (C) CD19⁺IL-10⁺ B cells and (D) CD19⁺TNFα⁺ B cells from HCs and CD patients are shown. (E) In addition, the ratios of Fr.I (IL-10⁺TNFα⁺ B cells) to Fr.II (IL-10⁺TNFα⁻ B cells) from HCs and CD patients are shown.