Fig. 3. Western blot analysis of vascular endothelial growth factor (VEGF), VEGF receptor (VEGFR)-1 and VEGFR-2 in retinal ganglion cell (RGC)-5 cells treated with bevacizumab. The expression of VEGF, VEGFR-1, and VEGFR-2 increased with the increase in bevacizumab concentration, and the expression of VEGF, VEGFR-1, and VEGFR-2 was increased in RGC-5 cells treated with H₂O₂. 1, control; 2, bevacizumab 1 mg; 3, H₂O₂ 800 µM; 4, bevacizumab 0.01 mg + H₂O₂ 800 µM; 5, bevacizumab 0.1 mg + H₂O₂ 800 µM; 6, bevacizumab 1 mg + H₂O₂ 800 µM.