Fig. 5.

DAF-16 is required for physical exertion-induced mitochondrial fusion. a ATP levels decrease during swimming. Graph of ATP levels (normalized to whole-animal protein levels) at the indicated times of continuous swimming. Error bars denote s.e.m. from two biological replicates, each with three technical replicates. P values were determined by Student’s t-test. b Average swim strokes per minute of 12 animals each for the indicated genotypes upon induction of swimming behavior. Error bars denote s.e.m. P values determined by Student’s t-test. c The percentages of muscle cells with elongated mitochondria in wild type, daf-16(mu86), and cand-1(tm1683); spg-7(ek25) animals for the indicated times post induction of swimming behavior. Full distributions of mitochondrial morphology and sample size (n) are shown in Supplementary Fig. 9. d DAF-16::GFP nuclear localization in body-wall muscle cells for animals at 0 and 300 min post induction of swimming behavior. White arrows indicate nuclei in the 0 min image. Scale bar, 10 µm. For all panels, asterisks above bars denote P value comparisons to wild type/controls; asterisks above lines denote comparisons under the lines: *P < 0.05; **P < 0.01; ***P < 0.001; ns = not significant