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. 2017 Aug 3;9:258. doi: 10.3389/fnagi.2017.00258

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Copyright © 2017 MacDonald, Barbat-Artigas, Cho, Peng, Shang, Moustaine, Carbonetto, Robitaille, Chalifour and Paudel.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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RNA and protein samples were prepared from nine WT and nine Egr-1−/− mouse diaphragm and soleus muscles. The western blots presented are representative blots from three independent experiments. (A) AGRN mRNA expression was significantly increased in Egr-1−/− mouse diaphragm when compared with WT. Similarly, in both diaphragm (B,C) and soleus muscles (D,E), the expression of agrin was significantly increased in Egr-1−/− mice compared with WT, with the exception of full length agrin in the soleus, which did not reach significance. All agrin protein bands were normalized to β-actin. Protein expression of neurotrypsin in the soleus was significantly decreased in Egr-1−/− mice compared with WT. All neurotrypsin protein bands were normalized to β-actin. p-values were obtained using Student’s t-tests or 2-way ANOVA, respectively. *(p ≤ 0.05); **(p ≤ 0.01); ***(p ≤ 0.001); ****(p ≤ 0.0001).