Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Aug 2;7:7163. doi: 10.1038/s41598-017-07295-5

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

PARTICLE transcripts are predominantly nuclear following irradiation and associated with chromosome 16 harbouring WWOX. (A,B) Representative epifluorescence microscopic images of U2OS sham irradiated (left) or 24 hr post 0.25 Gy (right) labelled with chromosome 16 paint (red: upper) and RNA in-situ hybridisation probes specific for PARTICLE (green: lower). Nuclei stained with DAPI (blue; lower) with merged images (upper). Mean fluorescence intensity analysis determined from regions-of-interest (ROI) indicated by circular dashed lines. Increased intensity over chromosome 16 indicated by arrows. (C,D) Plots of arbitrary units (AU) of overall PARTICLE (PART.) fluorescence intensity in the extra-nuclear (ExNc) plus nuclear (Nc) compartments (left) and intracellular distribution (right; green and blue lines represent PARTICLE and nuclear fluorescence respectively) in sham-irradiated (C) or 24 hr post 0.25 Gy (D). (E) Summary plots illustrating average relative fluorescence intensities pooled from the selected ROIs from the various experimental groups. (F) Scatterplot of arbitrary units (AU) of PARTICLE and chromosome 16 co-localization from a ROI (dashed circle), on (G) a representative high resolution epifluorescence micrograph after chromosomal spreading. (H) Summary co-localization plots for chromosome 16 (ChR = red channel) and PARTICLE (ChG = green channel) in ROIs taken from sham irradiated (0 Gy) or 24 hr post 0.25 Gy. Data are represented as mean ± SEM. (I) Representative epifluorescence microscopic images of U2OS labelled with RNA in-situ hybridisation probes specific for PARTICLE (PART: pseudo green: upper right) and dual labelled probe specific for a predicted Non-Triplex Region (NTR: pseudo red: lower left). Nuclei stained with DAPI (blue; upper left) with merged images (lower right). Arrow indicates absence of PARTICLE at NTR. (J) Scatterplot of arbitrary units (AU) of PARTICLE (PART.) and NTR indicating no co-localization. (K) Representative epifluorescence microscopic images of U2OS labelled with RNA in-situ hybridisation probes specific for PARTICLE (PART: pseudo green: upper right) and dual-labelled probe-specific for WWOX locus (pseudo red: lower left). Nuclei stained with DAPI (blue; upper left) with merged images (lower right). Arrow indicates presence of PARTICLE at WWOX locus. (L) Scatterplot of arbitrary units (AU) of PARTICLE (PART.) and WWOX indicating strong co-localization.