Fig. 1. T-DNA vector construction. A, T-DNA in the pPK2-NTC vector for nourseothricin selection; B, T-DNA in the pPK2-BAR vector for phosphinothricin selection; C, T-DNA in the pPK2-NTC-eGFP vector for nourseothricin resistance using green fluorescence for visual selection; D, T-DNA in the pPK2-BAR-mCherry vector with phosphinothricin resistance using cherry fluorescence for visual selection. PgpdA, promoter of glyceraldehyde-3-phosphate dehydrogenase gene from Aspergillus nidulans; NTC, nourseothricin resistance nat1 gene from Filobasidiella neoformans; Ptef, promoter of translation elongation factor gene from Aureobasidium pullulans; eGFP, enhanced green fluorescent protein gene; T, termination region of the glucoamylase gene from Aspergillus awamori; PtrpC, promoter of tryptophan C gene from Aspergillus nidulans; BAR, phosphinothricin resistance gene from Streptomyces hygroscopicus; TtrpC, terminator of tryptophan C gene from Aspergillus nidulans; mCherry, cherry fluorescent protein gene; RB and LB are the right and left border of T-DNA, respectively.