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. 2017 Aug 2;2(4):e00161-17. doi: 10.1128/mSphere.00161-17

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Copyright © 2017 Wangsanut et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.

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FIG 2 — The bas1Δ and grf10Δ mutants exhibit leaky adenine auxotrophy. (A) The wild-type (DAY286), BAS1 heterozygote (RAC105), bas1Δ (RAC108), and BAS1 complemented (RAC111) strains and GRF10 heterozygote (RAC114), grf10Δ (RAC117), and GRF10 complemented (RAC120) strains from C. albicans in the BWP17 background were grown overnight in YPD medium and were spotted at a starting OD₆₀₀ of 0.1 on plates containing SC agar medium with (+Ade) or without (−Ade) adenine. The plates were incubated for 48 h at 30°C. (B) The wild-type strain (OHWT), bas1Δ (TF016) and grf10Δ (TF021) mutant strains, and complemented (BAS1R and GRF10R) strains in the SN152 background were grown under the same conditions as in panel A.