FIG 6 .

Disruption of BAS1 mildly affects hyphal formation. To induce hyphae, wild-type and bas1Δ mutant strains were grown overnight in YPD medium and washed twice with sterile water. Cell densities were adjusted to 2 × 10⁷ CFU/ml, 5 µl of each strain was spotted onto YPD+10% serum, M-199, and Spider solid media, and plates were incubated for 3 days at 37°C and photographed. The induction of hyphae was performed at least three times, and representative examples are shown for each strain. Size bars, 500 μm.