TABLE 4 .
Name | Description | Sequence (5′→3′)a |
---|---|---|
BAS1-5DR | BAS1-pGEM3 sequence | CCAAATCCTCTGATGGTTTTATGCAACCCAGATTATTTTAGCATTCTAACTCGTATCAGCgttttcccagtcacgacgtt |
BAS1-3DR | BAS1-pGEM3 sequence | ACTACAATCAATCATCGTATATTCTTACATTAGCATCTGATTCTTATACACTAGAATACCtgtggaattgtgagcggata |
BAS1-DF | Diagnostic forward primer | GTGAAGTTTCTGATGCGAC |
BAS1-DR | Diagnostic reverse primer | GCCAAGGGACCTATTTGC |
B1RF | Restored allele forward primer | CTGGATCCATTGGCAGCATTATTG |
B1RR | Restored allele reverse primer | ACGGATCCACGCCTTAACCAACT |
G10RF | Restored allele forward primer | AGTGGGCCCCTTAGTATTCAACGA |
G10RR | Restored allele reverse primer | TGAGGGCCCGTATCATGACTTTG |
ADE1 | Forward primer | GAGACTATGCTGCTACTAAAGG |
Reverse primer | CAACACTTCGTCAACAAGAAC | |
ADE2 | Forward primer | CGATTCGGATCTACCAGTTATG |
Reverse primer | GGAGTTCTGTGTGCACTTAC | |
ADE4 | Forward primer | GTTGCCATGGCTAGAGAAG |
Reverse primer | TGGTGTCAGCTAAATCAATCC | |
ADE5,7 | Forward primer | CTCATATTACTGGTGGAGGATTAG |
Reverse primer | ATCTCTGGTACTTGCCATTG | |
ADE6 | Forward primer | GCAGCTGATATCCCTTCATTAG |
Reverse primer | TCCATACCAATGGCTTGAA | |
ADE8 | Forward primer | CTTTGGAGAAGGCAGGAATC |
Reverse primer | CTCCATCTTGACCAGCTTTC | |
ADE12 | Forward primer | GGTCCATTCCCAACAGAAC |
Reverse primer | ATCCAACCAACCACATCTTC | |
ADE13 | Forward primer | ACAAGAAGGTGGCGATAATG |
Reverse primer | GTTTGTTGAGGAGCTCTACC | |
ADE17 | Forward primer | AACAAGGTGCTGTTGATTTG |
Reverse primer | CTCCTAAGCCGATAACCATAC | |
MTD1 | Forward primer | TGTCCCATCCATTGGTAAAG |
Reverse primer | AAGAGGTCGCATCAGAAAC | |
SHM2 | Forward primer | CAAATTGATGGTGCTAGAGTTG |
Reverse primer | CTAACTCCACCTGGAACTAAAG | |
MIS11 (ADE3) | Forward primer | AATGTATGGTGCTGGTGAAG |
Reverse primer | GTCTTGGCGATACAGATTGG | |
NUP | Forward primer | GACCACCTCCATCAATGTC |
Reverse primer | TTGGAGTACCAGCAATAACC | |
TEF1 | Forward primer | TTCGTCAAATCCGGTGATG |
Reverse primer | CTGACAGCGAATCTACCTAATG |
Lowercase represents the nucleotides that anneal with the vector.