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. 2005 Feb 10;24(5):1033–1045. doi: 10.1038/sj.emboj.7600560

Figure 2.

Figure 2

The poly(A) tail of the EDC1 mRNA does not change in strains deficient in mRNA deadenylation. (A) A polyacrylamide Northern gel probed with oRP1211 is shown after cleavage of the EDC1 mRNA with oligo o3′ (oRP1209) without (−) or with (dT) subsequent oligo(dT) cleavage. Deletion strains are marked across the top above each bracket set. WT=yRP840; pop2Δ=yRP1920; ccr4Δ=yRP1616; ccr4Δ pan2Δ=yRP1620; pan2Δ=yRP1619; ski2Δ=yRP1195. M represents the marker lane the band sizes of which are shown on the left. Lanes are numbered across the bottom for reference. (B) The MFA2 mRNA probed with oRP140 in the same deletion strains as panel A. Marker sizes are given on the left. (C, D) The 7s standard probed with oRP100 for Northerns in parts A and B, respectively.