Skip to main content
. 2005 Feb 10;24(5):1033–1045. doi: 10.1038/sj.emboj.7600560

Figure 7.

Figure 7

The not2Δ, not4Δ, and not5Δ strains show alterations in the decay of the EDC1 mRNA. (A) Steady-state levels of the EDC1 mRNA in wild-type (WT) (yRP840), not2Δ (yRP1670), not3Δ (yRP1671), not4Δ (yRP1672), and not5Δ (yRP1673) strains. Strains are given across the top of the agarose Northern. The upper panel is probed for the EDC1 mRNA, and the lower panel shows the standardizing 7s probe. Values determined for the %mRNA based on multiple experiments are given between the two panels. (B) Half-life measurements in temperature-sensitive RNA polymerase II (rpb1-1) mutant strains. Wild-type (WT) yRP582 or not4Δ yRP1806 strains containing a temperature-sensitive rpb1-1 mutation were shifted to 37°C and RNA was analyzed at various times in minutes following the shift, which are labeled above each panel set. The top panel shows the half-life of the EDC1 mRNA. The middle panel gives the 7s standardizing mRNA. The bottom panel shows the endogenous MFA2 mRNA to demonstrate the effectiveness of the rpb1-1 shut off. Half-life values for each set of experiments are given on the right of the respective panels.