TABLE 3.
Oligonucleotide primers used in the PCR to amplify the desired DNA fragments
| Amplified fragment | Primer sequence (5′→3′)a |
|---|---|
| LamC | F1, ATGGCGACGAGAGCCGTGAAGG |
| R1, CTAGCGCCACTGGTAGGCG | |
| rLamC-ΔC | F2, CCGGAATTCCTGAAGGCGTGCGCAACGCAG |
| R2, CCGCTCGAGCTAGAAGGAGAACGCCTCCCAGC | |
| rLamC-ΔN | F3, CCGGAATTCTGGGCCGACGAGTTCGAC |
| R3, CCGCTCGAGCTAGCGCCACTGGTAGGCG | |
| rLamC | F4, CCGGAATTCGCTTCGCGGGACGCGGCGC |
| R4, CCGCTCGAGCTAGCGCCACTGGTAGGCG | |
| rLamC-Mutantb | F5, GCGGGGCGCTCGACATCATGGCGAACGTCG |
| R5, CGACGTTCGCCATGATGTCGAGCGCCCCGC |
a
Underlined sequences within the primers are the EcoRI and XhoI restriction sites.
b
The two mutated nucleotides are underlined and in boldface.