FIG 2.

Activation of the late promoter P_comGA by constitutive comX overexpression. (A) Maximum specific luciferase (Lux) activity (RLU OD₆₀₀⁻¹) emitted by eight independent clones (clones 1 to 8) of the ComX⁺ strain [BLD101(pGIBLD001), P_comGA-luxAB] compared to the control strain (Ctl⁻) carrying the empty vector [BLD101(pG⁺host9)]. (B) Kinetics of specific Lux activity (solid lines) during growth (OD₆₀₀, dotted lines) for the control strain (Ctl⁻, black lines) and three selected ComX⁺ clones [BLD101(pGIBLD001), clones [cl.] 2, 4, and 5; gray lines]. Cells were grown in CDMG. The results of one representative experiment of two independent replicates are shown.