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. 2017 Aug 1;83(16):e01074-17. doi: 10.1128/AEM.01074-17

FIG 5.

FIG 5

Activation of the late promoter PcomGA by inducible comX expression. (A) Kinetics of specific Lux activity during growth (OD600) of strain BLD101(pGIFPT001) grown in CDMG, CDMX, M17G, and M17X. The symbols for OD600 in CDMG (OD CDMG), CDMX (OD CDMX), M17G (OD M17G), and M17X (OD M17X) are long black dashes, long gray dashes, short black dashes, and short gray dashes, respectively. The symbols for Lux activity in CDMG (Lux CDMG) and M17G (Lux M17G) are solid black lines; the symbols in CDMX (Lux CDMX) and M17X (Lux M17X) are circles and squares, respectively. One representative experiment of three independent replicates. (B) Kinetics of specific Lux activity (solid lines) during growth (OD600, dashed lines) of five randomly selected clones of strain BLD101(pGIFPT001) grown in M17G and M17X. The symbols for OD600 in M17G (OD M17G) and M17X (OD M17X) are black and gray dashes, respectively. The symbols for Lux activity in M17G (Lux M17G) and M17X (Lux M17X) are gray and black solid lines, respectively. (C) Maximum specific luciferase (Lux) activity (RLU OD600−1) emitted by strains BLD101(pG+host9) (Ctl−, negative control, white bars), BLD101(pGIFPT001) (Pxyl, gray bars), and BLD101(pGIBLD001) (clone 2; P32, black bars) grown in CDMG, CDMX, M17G, and M17X. Mean values (n = 3) ± the standard deviations are indicated.