FIG 3.

Infection with multitoxin deletion mutants did not reduce excretion levels below those of WT infection. At 24 hpi, the water from fish infected with both Δcore and Δatt mutants showed similar levels of optical density (A), total protein (B), and mucin (C) as the water from WT V. cholerae-infected fish. These data were compiled from three experiments. Data shown are means ± standard errors of the means (SEM). One-way ANOVA was performed, followed by Tukey's multiple-comparison test to detect differences between groups. †, significant difference from the PBS group (P < 0.0001); ‡, significant difference from the HK group (P < 0.0001); **, significant difference between PBS and HK groups (P ≤ 0.01).