FIG 1.
PCR detection assay results for Vibrio isolates from Vietnam and Thailand. Numbers represent individual bacterial isolates. V. parahaemolyticus 5HP (VPAHPND) and VPS02 (VPnon-AHPND) from Thailand were included as positive and negative controls, respectively. (a) PCR amplicons for V. parahaemolyticus toxR; (b) AP4 nested PCR showing the expected AHPND results for all isolates except XN87; (c) duplex PCR for both the pirAVp and pirBVp genes showing the absence of pirAVp for XN87 only. Lanes M, DNA marker (2-log DNA ladder; New England BioLabs); lanes −ve, no-template control.