FIG 5.
Expression and quantification of the PirVp toxins. (a) Protein (20 μg) from the 80% ammonium sulfate precipitate fractions of the culture broths of the tested V. parahaemolyticus isolates was stained with Coomassie brilliant blue R-250 (left) and probed with monoclonal antibodies specific to the PirAVp and PirBVp proteins (right). Lane P, prestained protein marker (Thermo Scientific). (b) An indirect ELISA was performed to quantify the PirBVp protein from protein precipitates using serially diluted recombinant PirBVp toxin protein to make a standard curve (left). One microgram of the 80% protein fraction from each bacterial isolate was subjected to quantification, and the amounts of PirBVp are shown in the table (right). The gray triangle in the plot on the left represents the measurement for PirBVp in 5HP protein precipitates.