TABLE 2.
Primers used in this study
| Primer name | Primer sequence (5′-3′) | Target | Product size (bp) | Reference or source |
|---|---|---|---|---|
| toxR-F | ACGCAATCGTTGAACCAGAA | V. parahaemolyticus toxR | 359 | This study |
| toxR-R | ATACGAGTGGTTGCTGTCATG | 26 | ||
| PirAB-2020F | GCACCGTAAATTTTCAGGTT | Tandem pirABVp genes and a portion of their upstream and downstream regions | 2,020 | This study |
| PirAB-2020R | CGTTGCAATCTAAGACATAG | |||
| VpPirA-F | ATGAGTAACAATATAAAAC | pirAVp ORF | 336 | This study |
| VpPirA-R | TTAGTGGTAATAGATTG | |||
| VpPirB-F | ATGACTAACGAATACGT | pirBVp ORF | 1,317 | This study |
| VpPirB-R | CTACTTTTCTGTACCAA | |||
| VpPirA-F and VpPirB-R | Tandem pirABVp ORFs | 1,665 | This study | |
| VpORF70-F | CGTTGGCATCGTTCGTGTTG | ORF70 trypsin family protein | 326 | This study |
| VpORF70-R | TACCGTAAGTAGAGCGTTCA | |||
| VpORF75-F | CCGATACAAACTCACCACCA | ORF75 type II secretion protein | 429 | This study |
| VpORF75-R | AGGAGAAGTAGGGCAAAGAC | |||
| VpORF78-F | GATTGGCGTCAGTCAGTTCA | ORF78 metalloprotease RseP | 452 | This study |
| VpORF78-R | GCATCGCTTTGCTTTCTTTG | |||
| VpORF79-F | CGCCGCCTGATTGACACCTA | ORF79 type II and III secretion protein | 232 | This study |
| VpORF79-R | TGCCATCCCATTTCCCCGTA |