FIG 6.
Neighbor-joining tree of aligned amino acid sequences of FolPSMZ B27 and bacterial dihydropteroate synthases (DHPSs). The alignment was performed with prototypic DHPSs and sulfonamide-resistant DHPS variants (Sul1, Sul2, and Sul3) using the ClustalW program within MEGA 7. Genera and species are followed by their GenBank accession numbers in parentheses. DHPSs from the candidate phyla radiation (CPR) group bacteria are specified with black circles. Some of the branches were grouped to improve the visibility of the tree (the number of sequences grouped is indicated in parentheses). Bootstrap values were calculated as a percentage of 1,000 replicates, and those above 60% are shown at the branching points. The sequence of FolPSMZ B27 obtained in this study is depicted in red. Scale bar = 0.05 change/site.