TABLE 1.
Metagenomic fosmid libraries constructed using DNA extracted from antibiotic-exposed and control field plot soils
| Library name | No. of annual applications (expt period) | Antibiotic composition (application concn,a mg kg−1 soil) | No. of clones | Avg insert size (kb) | Cloned bases (Mb) |
|---|---|---|---|---|---|
| UTC | 0 (2010–2014) | None | 111,000 | 28 | 3,100 |
| MACRO | 5 (2010–2014) | Erythromycin (10), azithromycin (10), clarithromycin (10) | 593,000 | 30 | 17,900 |
| SCT | 16 (1999–2014) | Sulfamethazine (1, 10), chlortetracycline (1, 10), tylosin (1, 10) | 488,000 | 31 | 15,100 |
| Total | 1,192,000 | 36,100 |
a
For the SCT treatment, the annual application concentration for each drug was increased from 1 to 10 mg kg−1 soil since 2005.