TABLE 1.
S. cerevisiae strains used in this study
| Strain | Relevant genotype | Description/use | Reference |
|---|---|---|---|
| CEN.PK113-7D | MATa | Reference strain | 39 |
| CEN.PK113-5D | MATa ura3-52 | Uracil-auxotrophic reference strain | 39 |
| S288C | MATa | Marker strain for CHEF analysis | YGSCa |
| IMX585 | MATa can1::CAS9-tagA-loxP-natNT2-loxP | CEN.PK113-7D expressing Cas9 | 34 |
| IMS0478 | MATa, evolved | CEN.PK113-7D evolved for biotin prototrophy (accelerostat A) | This study |
| IMS0480 | MATa, evolved | CEN.PK113-7D evolved for biotin prototrophy (accelerostat B) | This study |
| IMS0481 | MATa, evolved | CEN.PK113-7D evolved for biotin prototrophy (accelerostat C) | This study |
| IMS0496 | MATa, evolved | CEN.PK113-7D evolved for biotin prototrophy (sequential batch reactor) | This study |
| IME327 | MATa ura3-52 pUDE446 | CEN.PK113-5D pUDE446 (BIO1-BIO6) | This study |
| IME329 | MATa ura3-52 pUDE448 | CEN.PK113-5D pUDE448 (BIO6) | This study |
| IME331 | MATa ura3-52 pUDE450 | CEN.PK113-5D pUDE450 (BIO1) | This study |
| IME334 | MATa ura3-52 p426GPD | CEN.PK113-5D p426GPD (empty) | This study |
| IMK129 | MATa ura3-52 tpo1::loxP-kanMX-loxP | CEN.PK113-5D; tpo1::loxP-kanMX-loxP | This study |
| IMZ694 | MATa ura3-52 tpo1::loxP-kanMX-loxP pUDE450 | IMK129 (tpo1Δ) pUDE450 (BIO1) | This study |
| IMZ695 | MATa ura3-52 tpo1::loxP-kanMX-loxP p426GPD | IMK129 (tpo1Δ) p426GPD (empty) | This study |
| IMK163 | MATa ura3-52 pdr12::loxP-kanMX-loxP | CEN.PK113-5D; pdr12::loxP-kanMX-loxP | This study |
| IMZ704 | MATa ura3-52 pdr12::loxP-kanMX-loxP pUDE450 | IMK163 (pdr12Δ) pUDE450 (BIO1) | This study |
| IMZ705 | MATa ura3-52 pdr12::loxP-kanMX-loxP p426GPD | IMK163 (pdr12Δ) p426GPD (empty) | This study |
| IMK773 | MATa ura3-52 tpo1::loxP-kanMX-loxP pdr12::hphNT1 | CEN.PK113-5D tpo1::loxP-kanMX-loxP pdr12::hphNT1 | This study |
| IMZ701 | MATa ura3-52 tpo1::loxP-kanMX-loxP pdr12::hphNT1 pUDE450 | IMK773 (tpo1Δ, pdr12Δ) pUDE450 (BIO1) | This study |
| IMZ702 | MATa ura3-52 tpo1::loxP-kanMX-loxP pdr12::hphNT1 p426GPD | IMK773 (tpo1Δ pdr12Δ) p426GPD (empty) | This study |
a
YGSC, Yeast Genetic Stock Center, Berkeley, CA.