TABLE 2.
Specific growth rates of laboratory-evolved biotin-prototrophic S. cerevisiae strains and of strains carrying defined mutations grown in shake flask cultures containing synthetic media with or without biotin supplementationa
| Strain | Relevant genotype | Growth rate (h−1) in SMD |
|
|---|---|---|---|
| Without biotin | With biotin | ||
| CEN.PK113-7D | Haploid laboratory strain | <0.01 | 0.39 ± 0.01b |
| IMS0478 | CEN.PK113-7D evolved for biotin prototrophy (accelerostat A) | 0.25 ± 0.00 | 0.34 ± 0.02 |
| IMS0480 | CEN.PK113-7D evolved for biotin prototrophy (accelerostat B) | 0.31 ± 0.00 | 0.34 ± 0.00 |
| IMS0481 | CEN.PK113-7D evolved for biotin prototrophy (accelerostat C) | 0.36 ± 0.00 | 0.41 ± 0.00 |
| IMS0496 | CEN.PK113-7D evolved for biotin prototrophy (SBR) | 0.32 ± 0.01 | 0.31 ± 0.02 |
| IME327 | CEN.PK113-5D + pUDE446 (2μ BIO1-BIO6) | 0.16 ± 0.01 | 0.32 ± 0.01 |
| IME329 | CEN.PK113-5D + pUDE448 (2μ BIO6) | No growth | 0.30 ± 0.00 |
| IME331 | CEN.PK113-5D + pUDE450 (2μ BIO1) | 0.15 ± 0.02 | 0.32 ± 0.01 |
| IME334 | CEN.PK113-5D + p426GPD (empty plasmid) | No growth | 0.34 ± 0.01 |
| IMZ694 | tpo1Δ, pUDE450 (2μ BIO1) | 0.23 ± 0.00 | 0.32 ± 0.00 |
| IMZ695 | tpo1Δ p426GPD (empty plasmid) | No growth | 0.33 ± 0.01 |
| IMZ704 | pdr12Δ pUDE450 (2μ BIO1) | 0.25 ± 0.00 | 0.37 ± 0.00 |
| IMZ705 | pdr12Δ p426GPD (empty plasmid) | No growth | 0.40 ± 0.00 |
| IMZ701 | pdr12Δtpo1Δ pUDE450 (2μ BIO1) | 0.25 ± 0.01 | 0.36 ± 0.01 |
| IMZ702 | pdr12Δtpo1Δ p426GPD (empty plasmid) | No growth | 0.37 ± 0.00 |
a
Cells were grown in aerobic 100-ml shake flask cultures. The data represent averages ± standard deviations of the mean of measurements of independent duplicate cultures.
b
Reference 32.