Figure 3.

FHL2 suppresses FOXO1-induced apoptosis. (A) DU145 cells were transfected with pCMV-CD20 (3 μg), Flag-FOXO1 (2.5 μg), and Myc-FHL2 (2.5 μg) as indicated. At 16 h post-transfection, CD20-positive cells were separated from CD20-negative cells by FACS-based sorting. The apoptotic index of CD-20 positive cells was determined as described in Materials and Methods. Representative profiles are shown in the top graphs. Apoptotic indexes from four independent experiments are shown in the table below the graphs. (B) DU145 cells were transfected with FOXO1 (4 μg) and FHL2 (4 μg) as indicated. The activity of capase-3 was determined as described in Materials and methods.