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. 2017 Aug 1;83(16):e00953-17. doi: 10.1128/AEM.00953-17

TABLE 2.

qPCR primers and probes utilized in this study

Target Primer or probe sequence (5′–3′)a Reference
Pan-pneumococcus lytA F, ACGCAATCTAGCAGATGAAGCA 43
R, TCGTGCGTTTTAATTCCAGCT
FAM-GCCGAAAACGCTTGATACAGGGAG
Serotype 4 F, TGGGATGACATTTCTACGCACTA 47
R, CCGTCGCTGATGCTTTATCA
FAM-TCCTATTGGATGGTTAGTTGGTGA
Serogroup 6 F, AAGTTTGCACTAGAGTATGGGAAGGT 47
R, ACATTATGTCCRTGTCTTCGATACAAG
FAM-TGTTCTGCCCTGAGCAACTGG
Serotype 19F F, GGTCATGCGAGATACGACAGAA 47
R, TCCTCATCAGTCCCAACCAATT
FAM-ACCTGAAGGAGTAGCTGCTGGAACGTTG
Serotype 23F F, TGCTATTTGCGATCCTGTTCAT 47
R, AGAGCCTCCGTTGTTTCGTAAA
FAM-TTTCTCCGGCATCAAACGTTAAG
comC gene (JVS71L/JVS72R) F, CCTTTACAAATAAAATGGTAACTGTG This study
R, AATGCTCTATCCAGCTGAGCTAT
a

qPCR was conducted using primer and probe concentrations of 100 nM each for lytA qPCR and 200 nM each for serotype-specific qPCR. Forward (F) and reverse (R) primer sequences are shown for each target, followed by the specific probe sequence. All probes were labeled at the 5′ end with 6-carboxyfluorescein (FAM) and also with black hold quencher 1 dye at the 3′ end.