TABLE 2.
qPCR primers and probes utilized in this study
| Target | Primer or probe sequence (5′–3′)a | Reference |
|---|---|---|
| Pan-pneumococcus lytA | F, ACGCAATCTAGCAGATGAAGCA | 43 |
| R, TCGTGCGTTTTAATTCCAGCT | ||
| FAM-GCCGAAAACGCTTGATACAGGGAG | ||
| Serotype 4 | F, TGGGATGACATTTCTACGCACTA | 47 |
| R, CCGTCGCTGATGCTTTATCA | ||
| FAM-TCCTATTGGATGGTTAGTTGGTGA | ||
| Serogroup 6 | F, AAGTTTGCACTAGAGTATGGGAAGGT | 47 |
| R, ACATTATGTCCRTGTCTTCGATACAAG | ||
| FAM-TGTTCTGCCCTGAGCAACTGG | ||
| Serotype 19F | F, GGTCATGCGAGATACGACAGAA | 47 |
| R, TCCTCATCAGTCCCAACCAATT | ||
| FAM-ACCTGAAGGAGTAGCTGCTGGAACGTTG | ||
| Serotype 23F | F, TGCTATTTGCGATCCTGTTCAT | 47 |
| R, AGAGCCTCCGTTGTTTCGTAAA | ||
| FAM-TTTCTCCGGCATCAAACGTTAAG | ||
| comC gene (JVS71L/JVS72R) | F, CCTTTACAAATAAAATGGTAACTGTG | This study |
| R, AATGCTCTATCCAGCTGAGCTAT |
a
qPCR was conducted using primer and probe concentrations of 100 nM each for lytA qPCR and 200 nM each for serotype-specific qPCR. Forward (F) and reverse (R) primer sequences are shown for each target, followed by the specific probe sequence. All probes were labeled at the 5′ end with 6-carboxyfluorescein (FAM) and also with black hold quencher 1 dye at the 3′ end.