TABLE 1.
Primers for construction of strains used in this work
| Primer | Sequence (5′ → 3′) |
|---|---|
| CODH_F | TCATTTAACATAGATAATTGGATCCGTAATTCAGCAAGGAGGG |
| CODH_R | ATTTATATAAAATTTATATTCCTAATTTTTTACGTTTTTCATTG |
| ACS_F | TAGGAATATAAATTTTATATAAATTTTAATTTTAGGGAGGG |
| ACS_R | GCGGATTCTAGATTGGATTACATTATTGGATCCATAGTTAATG |
| CODH/ACS_his_R | ACCGCGGATTCTAGATTGGATTAATGATGATGATGATGATGCATTATTGGATCCATAGT |
| p94_sbfI_f | AAAGCTCCTGCAGGTCGACTGTGGATGG |
| CODHonly_f | GTAAAAGAGATTGTTTCTAGCTC |
| CODHonly_r | TAAAATTTATATTCCTAATTTTTTACGTTTTTC |
| ACS_BamHI_f | GGTTTATCTGTTACCCCGTAGGATCCTAGGGAGGGGCAAAAAAA |
| ACS_XbaI_r | GATGCATGCTACCGCGGATTCTAGATGTCGTATTATAAGTTTTATTACATTATTG |
| p95_AcsF_f | CTAGAATCCGCGGTAGCATGCTGCATGACAATAGGAGGAG |
| p95_AcsF_r | ATCATTAAGTGGCGCCTTTACGCGTAGGAAGAACACTTTCTCC |
| p94_CODH/CooC_f | GTTAATCATTTAACATAGATAATTGGATCCAGGAGGGAATTTATTAAAATGG |
| p94_CODH/CooC _r | GTTAATCATTTAACATAGATAATTGGATCCAGGAGGGAATTTATTAAAATGG |
| p94_ACS_f | GGGGGTATTTTAAAGGGAGGGGCAAAAAAATG |
| p94_ACS_r | GGGGGTATTTTAAAGGGAGGGGCAAAAAAATG |