Figure 5.

Cell cycle analysis was carried out on GH3 cells transfected with AHR/EV or Ahr/Ctrl siRNA and treated with vehicle or BαP for 24 hours prior to fixation and flow cytometry. (A) Percentage of GH3 cells in G0/G1 phase in differently treated cells. Cell cycle analysis was done in triplicate and experiment was repeated three times using synchronized cells. (B) Cell Quest Pro software was used to gate the cell population and only gated cells were used for the final cell cycle analysis. Areas designated as belonging to G0/G1 (M1), S phase (M2) and G2 phase were assigned as shown. (*P<0.05, error bars represent standard error).