Figure 4.

Effect of WEF on the growth influenza A/PR/8/34 (H1N1) virus in RAW 264.7 cells. (A) Immunofluorescence analysis of influenza A M2 protein in cells. Cells were treated with WEF (10, 100 μg/mL) and IFN-β (1,000 U/mL) after influenza A virus infection. Influenza A virus M2-specific antibody was used for detection by fluorescence microscopy. Cells were also stained with DAPI, and the merged image shows the cytoplasmic location of the virus M2 protein (red). (B) RAW 264.7 cells were treated with WEF (10, 100 μg/mL) and IFN-β (1,000 U/mL) 12 h before infection with PR8-GFP (MOI 1). The antiviral effect was verified after 24 h of viral infection as the presence or absence of GFP expression.