Table 2.

Oligonucleotides used in the site-directed mutagenesis of Arn1p

Mutation	Oligonucleotide sequence
Y98A	5′-CGGTAACATCCGTTATATTGCCACTGGTTACG CCACATCATCCTATAGTGAGC-3′
Y101A	5′-CGGTAACATCCGTTATATTTACACTGGTGCCG CCACATCATCCTATAGTGAGC-3′
R132A	5′-GCAAATCATATACGCTGCGTTGTCTGATGTGT TTGG-3′
Y166A	5′-CACAAGCTTATGATGTTCAAAGAGCCGCTGCA GGTGCCATTTTCTACAATGC-3′
F208A	5′-GTCCCGACTTGGCCTGCTATCATCAACACATG GATTGC-3′
Y380A	5′-CTATATGGCCGCTGATGCTTTGTACACGGTTA TGATTGTTGCCGTC-3′
Y534A/Y538A	5′-GGTGATGTTGCTCTAGCCACAACTGCCGCCGA ATCTCCAGCTACTTTCATTGAAACCTATACTTGG- 3′
F540A/Y544A	5′-GCCTACGAATCTCCATATACTGCCATTGAAAC CGCTACTTGGGGTACCCCACAAAGGAATGCC-3′
QRYR-A	5′-CTTGGGGTACCCCAGCAGCGAATGCCTAATGA ACGCTGCCAAATACGTTCAAGCATTGGAAACTATC G-3′
3F-A	5′-CCAATTTTGGATTGGGCTGAAAAACTTCCATC AAAAGCCACTGCTAAACGGGAAGAACAAAAGCTGG -3′
Nucleotide substitutions resulting in alanine mutations are underlined.