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. 2005 Feb 17;24(5):1046–1056. doi: 10.1038/sj.emboj.7600580

Figure 6.

Figure 6

Hep1 prevents aggregation of mtHsp70 in E. coli cells. MtHsp70 was expressed in E. coli either alone (mtHsp70) or together with a His-tagged form of Hep1 (mtHsp70+Hep1). Both proteins lacked their matrix targeting sequences. Following induction of protein expression cells were lysed and one aliquot was removed (total, T). The other aliquot was separated by centrifugation into insoluble protein (P) and soluble protein fractions (SN). Samples were analysed by SDS–PAGE and Coomassie staining.