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. 2017 Aug 3;8:556. doi: 10.3389/fphys.2017.00556

Figure 8.

Figure 8

Regulatory mechanisms in cardiac fibroblasts. (A) By incubating cardiac fibroblasts with OPN, the mRNA expression of LOX and Col-III increased after 24 and 48 h, respectively. (B) OPN expression in cardiac fibroblasts could be induced by Ang-II and NAC. (C–E) Ang-II increased the expression of LOX and Col-III, however, Col-III expression depends on the presence of LOX (si-LOX, siRNA against LOX; sc-LOX, control siRNA). Data are means ± S.D. of n = 6 hearts. *p < 0.05 vs. control, #p < 0.05 vs. Ang-II. (F) Downregulation of OPN reduced the expression of LOX in both unstimulated and Ang-II stimulated cells after 48 h (si-OPN, siRNA against OPN). Data are means ± S.D. of n = 6 hearts. *p < 0.05 vs. control. (G) OPN mRNA was primarily elevated in left ventricular tissue of trained SHR. The expression of HPRT was used for normalization. Data are means ± S.D. of n = 6 animals. *p < 0.05 vs. Wistar sed., #p < 0.05 vs. SHR sed.