Effects of exogenous 2-(4-carboxyphenyl)-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide (c-PTIO) on the Cd accumulation in Arabidopsis plants. Six-day-old seedlings were grown on 1/2 MS agar medium with 50 μM CdCl2 after 12 d of exposure to 1 mM c-PTIO pulse SAY09 (+c-PTIO+SAY09), SAY09 (+SAY09) or 50 μM GSNO (+GSNO) treatments. Then, these plants were used to analyze the phenotypes (A), total chlorophyll content (B), shoot (C) and root (D) Fe content, the Cd concentration in shoots and roots (E), HC1 content (F), the Cd concentrations in HC1 (G) and cell wall (H) of roots, and the Fe concentration in HC1 (I) and cell wall (J) of roots. Different letters above each bar indicate significant difference at p < 0.05.