Table 1.
Primer sequences of the genes for rapid amplification of cDNA ends (RACE), full-length open reading frames (ORFs) and real-time PCR (qRT-PCR).
| Primer-ID | Forward PCR Primer (5′-3′) | Reverse PCR Primer (5′-3′) |
|---|---|---|
| ThSHR1_3OUTER | TGTGTGAGCGCCGTGAGAAGGCTGTGCA | TACCGTCGTTCCACTAGTGATTT |
| ThSHR1_3INNER | TTTTGCAGTTTTTTGTAAGTCCTG | CGCGGATCCTCCACTAGTGATTTCACTATAGG |
| ThSHR1_5OUTER | CTAATACGACTCACTATAGGGCAAGCAGTGGTATCAACGCAGAGT | ATCCATAGAAGATGCTGCATTCGC |
| ThSHR1_5INNER | CTAATACGACTCACTATAGGGC | GTTGGTGGTGGTATTGGGCTTCTC |
| ThSHR1_ORF | ATGGATAGATTGTTTACCTCCAGCATA | TTACAAGCAAGGCTTCCAGGCGG |
| ThSHR1_qRT-PCR | TACCCGCGACCTTCAGTGAC | CAGGCGGAAGCCCAAATGAC |
| ThSHR2_3OUTER | TTCTGTTATGAAGGAAATAGGGCA | TACCGTCGTTCCACTAGTGATTT |
| ThSHR2_3INNER | TCAGCCTGTGTTTCGTTTCGCTTGA | CGCGGATCCTCCACTAGTGATTTCACTATAGG |
| ThSHR2_5OUTER | CTAATACGACTCACTATAGGGCAAGCAGTGGTATCAACGCAGAGT | CTGAACTCAAACGGCACTCCCATAA |
| ThSHR2_5INNER | CTAATACGACTCACTATAGGGC | AGGGACTAGCCTCCTGGAATTTCAG |
| ThSHR2_ORF | ATGTCATATAGTTTTTATACCCATTC | CTATTTAGGTTTCCAGGCAGAAG |
| ThSHR2_qRT-PCR | ACTCAGTGGCCCATGCTGTT | AACGGCACTCCCATAAGCCT |
| ThSCR_3OUTER | ATCCTGATGACAGCATAGAAAGACA | TACCGTCGTTCCACTAGTGATTT |
| ThSCR_3INNER | TGGAACTCTCAAACTTGGCTGGAAAGAC | CGCGGATCCTCCACTAGTGATTTCACTATAGG |
| ThSCR_5OUTER | CTAATACGACTCACTATAGGGCAAGCAGTGGTATCAACGCAGAGT | ACATTGGAGTAGAAGATCCAGAAGG |
| ThSCR_5INNER | CTAATACGACTCACTATAGGGC | TCCAGTTTGCAGCTCCAATTTCATC |
| ThSCR_ORF | ATGACATTTGCTTCTTCTAAATTGTGTG | TTACAGTGCATCATGGGTGGGTGTC |
| ThSCR_qRT-PCR | ATGGCCAGGGCTATTCCACA | CTGCCTGTTGCCTCCAATGC |
| APRT_qRT-PCR | TCCACAGGTTCTTGAATCGCT | TGACTTGAGCCTCATTCGCTC |