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. Author manuscript; available in PMC: 2017 Aug 3.
Published in final edited form as: Virology. 2016 Oct 19;500:22–34. doi: 10.1016/j.virol.2016.09.029

Fig. 7.

Fig. 7

Increased protein stability in an E7-dependent manner contributes to the increased levels of BRCA1 and Rad51 in HPV31 positive cells. (A, B) Primary HFKs, HFKs maintaining wild type HPV31 genomes (HFK-31) or mutant HPV31 genomes with a mutation in the Rb binding site (HFK-31 ΔLHCYE), as well as (C, D) pLXSN-E7 and pLXSN E7 ΔLHCYE cells were treated with 50 μg/ml cycloheximide over a 12 h time course. Whole cell lysates were harvest at the indicated times using antibodies to BRCA1 and Rad51, with GAPDH serving as a loading control. (A, B) Data shown are representative blots from three independent experiments from two different HFK donors, and for (C, D) data shown are representative blots from three independent experiments from one HFK donor. (B, D) Graphed are the relative protein levels at each time point, with T0 for each cell line set to 100. Densitometry was performed across three independent experiments using Biorad ImageLab 5.0 software. Error bars represent means +/− standard error.