Table 2.

Measures and methods

Domain	Outcome measure	Methods/equipment
Demographics	Cardiovascular risk factors Cardiovascular disease Cardiovascular family history Drug use	Evaluation medical records Questionnaire
	Diabetes mellitus Renal function Lipid profile Liver function Thyroid function	Routine blood analysis, including random glucose, HbA1c, creatinine, total cholesterol, LDL, HDL, triglycerides, ALAT, ASAT, gamma-GT, LDH, TSH, T4 and hsCRP. EDTA, li-heparine tube, NaF tube.
	Weight	Seca 877 scale
AGEs in arterial wall	Quantitative measurement	High-performance liquid chromatography
	Types and localisation	Immunohistochemistry
AGEs in fluids	Serum AGEs	High-performance liquid chromatography
	Urine AGEs	High-performance liquid chromatography
Skin AGEs	Skin autofluorescence	AGE Reader
Vascular parameters	Blood pressure	Microlife WatchBP Office
	Ankle-brachial index	Vicorder
	Diameter aorta	Siemens Acuson S2000
	Intima-media thickness	Esaote MyLabOne
	Pulse wave velocity	Sphygmocor EM3

AGEs, advanced glycation end-products; ALAT, alanine aminotransferase; ASAT, aspartate aminotransferase; GT, glutamyltransferase; HDL, high-density lipoprotein; hsCRP, high sensitivity C-reactive protein; LDH, lactate dehydrogenase; LDL, low-density lipoprotein; TSH, thyroid-stimulating hormone.