(a) The sex-specific differential splicing pattern of Bmdsx in the embryonic stages of 78, 96 and 120 h. (b) Box plot of the log2-transformed FPKM distribution of autosomal (A, yellow) and Z-linked (Z, black) genes that showed expression (FPKM ≠ 0) in male and female samples. The boxes represent the interquartile range (IQR), the notch of box plots represents median (horizontal line) expression with a 95% confidence interval and the outliers were not plotted. The asterisk represents a significantly (MWU, p < 0.0001) reduced expression of Z-linked genes when compared with that of the autosomes.