Figure 8.

Validation of RNA-seq results of embryonic stages (78, 96 and 120 h) and head samples by qRT-PCR. rp49 was used as an endogenous reference expression. Female samples were used as calibrators; hence, the relative expression (M/F) of females can be taken as 1 for all the genes. The relative expression of selected five autosomal (a) and five Z-linked genes (b) for the three embryonic stages and four equally expressing (M/F = approximately 1) autosomal (c) and four Z-linked genes (d) were shown in the head samples.