Figure 1.

Control, constant acidification and oscillating acidification-exposed Chromis punctipinnis were individually placed in the light/dark preference test arena and their location recorded for 900 s. In January (n = 15–16), fish from all three treatments spent significantly more time in the dark zone than 450 s (‡p < 0.0001 in all cases), while in September (n = 17) they did not. Time spent in the dark zone was significantly affected by season (January versus September; F₁,₉₂ = 7.9540, p = 0.0059), but it was not significantly affected by CO₂/pH treatments (F₂,₉₂ = 0.3557, p = 0.7017). There were no significant interaction effects (F₂,₉₂ = 0.4629, p = 0.6309). Data presented as mean ± s.e.m.