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. 2017 Jul 3;6(7):e354. doi: 10.1038/oncsis.2017.56

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Copyright © 2017 The Author(s)

Oncogenesis is an open-access journal published by Nature Publishing Group. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Evaluation of vascular and lymphatic markers in biopsies from patients diagnosed with HNSCC. Representative images of immunohistochemistry for (a) PDPN and (b) CD31 expression: (1) oral and (2) hypopharyngeal localization; Left panels (1.a, 2.a)—primary tumor; Right panels (1.b, 2.b)—relapsed tumor in the same patient after surgery and chemo-X radiotherapy (brown, PDPN/CD31; blue, hematoxylin - nuclei); (c) quantitative PDPN, VEGF-C, LYVE1 and PROX1 mRNA expression, as percentage of control (0 Gy); * and **, significantly increased values (P<0.05 and P<0.01, respectively) post-, as compared to pre-X radiotherapy.