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. 2017 Jul 3;6(7):e353. doi: 10.1038/oncsis.2017.57

Figure 3.

Figure 3

JWA negatively regulates DR4 expression and sensitivity of GC cells to TRAIL. (a) JWA expression in GC cells BGC823 and SGC7901 and their variants was determined by western blotting analyses. (b) GC cells BGC823 and SGC7901 and their variants were transfected with si-JWA or Flag-JWA or a corresponding control for 48 h; DR4 and JWA expression in these cells was determined by western blotting analyses. (c) Left: Si-JWA or Flag-JWA GC cells or a corresponding control were transfected into BGC823 or its variant for 48 h; the DR4 surface expression was then analyzed by flow cytometry. Right: Comparison of relative DR4 surface expression between different groups. Respective DR4 surface expression was estimated as depicted in Figure 1b. Si-JWA transfected-BGC823 (d) and Flag-JWA transfected-BGC823/DDP cells (e) were exposed to the indicated TRAIL concentrations for another 24 h; JWA and DR4 expression and the apoptotic biomarkers cleaved-PARP-1 and cleaved-caspase-8, -9 and -3 were determined by western blotting analyses. The experiments were independently performed at least three times, and representative data are shown. For the flow cytometric assay, the data are shown as the means±s.e.m., *P<0.05.