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. 2017 Jul 24;6(7):e363. doi: 10.1038/oncsis.2017.63

Figure 3.

Figure 3

ZNF687 upregulation promotes HCC CSC-like traits in vitro. (a) GSEA plot showing that high ZNF687 expression correlated positively with stem cell gene signatures in published TCGA expression profiles of patients with HCC. (b) Western blot analysis of ZNF687 expression in Huh7 and Hep3B cells stably overexpressing ZNF687. (c) Representative micrographs of tumorspheres formed. Scale bar, 100 μm. Histogram shows the mean number of tumorspheres formed. (d) Hoechst 33342 dye exclusion assay showing that ZNF687 overexpression promoted the SP+ subpopulation. (e) Flow cytometry analysis of the CD133+ population in Huh7 and Hep3B cells stably overexpressing ZNF687. (f) Western blot analysis of ZNF687 expression in SP+ or SP− cells and CD133+ or CD133− cells. (g) Real-time PCR of mRNA expression of the pluripotency-associated factors. Transcript levels were normalized to glyceraldehyde-3-phosphate dehydrogenase (GAPDH) expression. Error bars, mean±s.d. of three independent experiments. *P<0.05.