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. 2017 Jul 24;6(7):e363. doi: 10.1038/oncsis.2017.63

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Copyright © 2017 The Author(s)

Oncogenesis is an open-access journal published by Nature Publishing Group. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Silencing ZNF687 inhibits HCC stem cell-like traits in vitro. (a) Western blot analysis of ZNF687 expression in ZNF687-silenced Huh7 and Hep3B cells. (b) Representative micrographs of tumorspheres formed. Scale bar, 100 μm. Histogram shows the mean number of tumorspheres formed. (c) Hoechst 33342 dye exclusion assay showing that silencing ZNF687 decreased the SP⁺ subpopulation. (d) Flow cytometry analysis of the CD133⁺ population in Huh7 and Hep3B cells stably expressing ZNF687-shRNA(s). (e) Real-time PCR of the mRNA expression of the pluripotency-associated factors. Transcript levels were normalized to GAPDH expression. Error bars, mean±s.d. of three independent experiments. *P<0.05. RNAi, RNA interference.