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. 2017 Jul 24;6(7):e363. doi: 10.1038/oncsis.2017.63

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Copyright © 2017 The Author(s)

Oncogenesis is an open-access journal published by Nature Publishing Group. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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ZNF687 targets the BMI1, NANOG and OCT4 promoters. (a) ChIP analysis of the physical association of regions of the target gene promoters with ZNF687. ChIP assays were performed using a FLAG antibody to identify ZNF687-binding sites in the target gene promoters. IgG, negative control. (b) Luciferase reporter gene assays of the target gene promoters. Renilla luciferase activity, normalized control. (c) Western blot analysis of BMI1, NANOG and OCT4 protein expression in Huh7 and Hep3B cells stably overexpressing ZNF687 or in which ZNF687 had been silenced. α-Tubulin, loading control. (d) Quantification of tumorspheres formed in ZNF687-overexpressing cells treated with BMI1, NANOG or OCT4 small interfering RNA (siRNA). (e) Hoechst 33342 dye exclusion was used to examine the effects of BMI1, NANOG or OCT4 knockdown on the proportion of SP⁺ cells in ZNF687-overexpressing cells. Bars, mean±s.d. of three independent experiments. *P<0.05.