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. 2017 Jul 31;6(7):e365. doi: 10.1038/oncsis.2017.68

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Copyright © 2017 The Author(s)

Oncogenesis is an open-access journal published by Nature Publishing Group. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Expression of a constitutively active MKK7 mutant restores melanoma growth and migration. (a) Immunoblotting for pc-Jun, c-Jun, β1-integrin and Actin. Protein lysates were collected from A2058 cells transduced for expression of shCon, shMALT1 either with or without the constitutively active MKK7 mutant, and then treated with or without TNFα. (b) Soft agar colony assay. Cells transduced as in (a) were used for soft agar colony assay, and colony numbers were counted at the 2-week time-point. Graph represents average number of colonies ±s.d. P-values of less than 0.05 were obtained via two-tiered Student-T-test. (c) Cell migration via scratch-wound assay. Transduced A2058 cells were grown to near confluence, and then incubated with low serum media (0.1% FBS) for overnight and then scratch-wounded. Images were taken at 0 and 24 h time-points.