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. 2001 Jul 17;98(16):9318–9323. doi: 10.1073/pnas.161204098

Figure 4.

Figure 4

i.n. immunization with protein σ1-PL-DNA elicits Th2 cell-dependent responses. BALB/c mice were i.n. immunized 3 times at weekly intervals with either protein σ1-PL-pCMV-β-gal (A–C) or pCMV-β-gal (D–F), and 4 weeks subsequent the last dose, mononuclear cells from CLN (A, D), PP (B, E), and spleens (C, F) were harvested and left unrestimulated (open bars) or restimulated with 80 μg/ml β-gal (filled bars) for 2 days. CFCs were enumerated by cytokine-specific ELISPOT assay. β-gal restimulation of protein σ1-PL-DNA vaccinated mice induced increases in CLN IL-4, IL-5, and IL-6, PP IL-4 and IL-6, splenic IL-6, and no IFN-γ CFC in any of the tissues. β-gal restimulation of DNA vaccinated mice showed increases in only CLN IL-6, PP IL-4 and IL-6, and splenic IL-6 CFC. Data are representative of three experiments (± SD). *, P ≤ 0.007; **, P = 0.021.