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. 2017 Jul 7;28(14):1853–1861. doi: 10.1091/mbc.E17-01-0034

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© 2017 Fong et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 3: — Removal of calmodulin from the nuclear-side tether weakens SPB–microtubule attachments. (A) Individual rupture force traces for wild-type SPBs, Spc72-AID SPBs, Spc110-407 SPBs, and kinetochores. Gray dots show raw data. Colored traces show the same data after smoothing (500-ms sliding boxcar average). Dashed vertical lines mark start of the force ramp. Arrows mark ruptures at SPB–microtubule or kinetochore–microtubule interface. (B) Estimated survival probability as a function of force for microtubule attachments to wild-type and Spc72-AID SPBs (red and yellow curves, respectively). Shaded area shows 95% confidence interval for the Spc72-AID curve. Dashed vertical line marks the estimated force at 75% survival for Spc72-AID. The curve for Spc72-AID is not significantly different from wild type, indicating that ablation of the cytoplasmic face of the SPB does not affect the measured strengths. (C) Estimated survival probability as a function of force for indicated attachments. Shaded areas show 95% confidence intervals. Dashed vertical lines mark estimated forces at 75% survival for kinetochores and Spc110-407 SPBs (gray and blue, respectively). Survival for Spc110-407 is reduced significantly compared with wild type. Measurement statistics and p values are summarized in Table 1. Wild-type data in A–C are recopied from Figure 2, C and E, for comparison.