Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Jul 7;28(14):1871–1882. doi: 10.1091/mbc.E17-01-0014

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2017 Herum et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

“ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society for Cell Biology.

PMC Copyright notice

FIGURE 2: — Paracrine signaling from stretched CMs induces CFB proliferation. (A) Schematic illustration of the principles of the paracrine signaling model. (B) Proliferation rate of CFB cultures alone or in coculture with nonstretched CMs or stretched CMs. CFBs were plated on PA gels with 8 kPa stiffness. Two-way ANOVA showed significant effect of culture type*** and Tukey’s post hoc test as indicated in figures. N = 3–6. (C) Proliferation rate of CFBs in CM coculture or treated with conditioned media from cocultures. Two-way ANOVA showed an effect of culture type***, conditioned media***, and a significant interaction**. Tukey’s post hoc test results are indicated in figures. N = 3. (D) Relative expression of chemokine (C-X-C motif) ligand 1 (CXCL1), CSF-1, interleukin-1 receptor antagonist (IL-1ra), cluster of differentiation 54 (CD54), PDGF-B, and FGF2 mRNA normalized to GAPDH mRNA in CMs stretched for 4 and 24 h. *Denotes significant difference from 0 h control as determined by Student’s t test. N = 9 (control) and 4 (stretch). (E) Proliferation rate following 24 h stimulation with recombinant CSF-1 and/or PDGF-B. One-way ANOVA showed significant effect of stimulation*** and Tukey’s post hoc test results as indicated. N = 3. (F) Relative proliferation rate of CFBs in cocultures in the presence of PDGF-B and CSF-1 receptor antagonists (AG and GW, respectively). Two-way ANOVA showed an effect of culture type*** and blockers*** as well as significant interaction* between the two. Tukey’s post hoc test results are indicated in the figure. N = 3. Cell area (G) and morphology (H) of CFBs on 8 kPa alone or in coculture with nonstretched and stretched CMs. One-way ANOVA showed significant effect of culture type* on cell area and Tukey’s post hoc test results as indicated in figure. *P < 0.05, **P < 0.01, ***P < 0.001.