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. 2017 Jul 7;28(14):1871–1882. doi: 10.1091/mbc.E17-01-0014

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© 2017 Herum et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 4: — Matrix stiffening causes cell spreading, SMA fiber formation, up-regulation of collagen I, and down-regulation of collagen III. (A) Schematic illustration of the principles of the stiffening model. (B) immunofluorescence staining for SMA (green). F-actin and nuclei were stained with phalloidin (red) and DAPI (blue), respectively. (C) Collagen (col) 1a1, 1a2, and 3a1, fibronectin (FN1), LOX, and OPN mRNA normalized to GAPDH mRNA in CFBs on 8 kPa gels stiffened to 30 kPa. N = 4 (control) and 12 (stiffened). (D) Immunofluorescence staining for FN (green) and quantification of staining intensity 48 h after gels stiffening. N = 8. F-actin was stained with phalloidin (red). Student’s t tests were applied to determine significant changes. *P < 0.05; n.s., nonsignificant.