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. 2017 Jul 7;28(14):1924–1936. doi: 10.1091/mbc.E16-12-0875

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© 2017 Connacher et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 2: — Dynamic movement of WRAMP structures, followed by membrane retraction. Frames from confocal live-cell imaging experiments of (A) WM239a and (B) A375 cells cotransfected with MCAM-GFP and LifeAct-mCherry and (C) WM239a and (D) A375 cells cotransfected with MCAM-GFP and myosin IIB-N18-mCherry. Supplemental Movies S2–S5 (corresponding to A–D, respectively) show coordinated movement of MCAM, F-actin, and myosin IIB. White dot indicates starting position. Scale bars, 10 µm; times in hours:minutes. Controls for this experiment with MCAM-GFP plus mCherry in Supplemental Figure S1 and Supplemental Movies S6 and S7. Quantitation of MCAM-GFP and myosin IIB-mCherry (or LifeAct-mCherry) fluorescence vs. time is shown in Supplemental Figure S2.