FIGURE 2:

Myosin and actin colocalize into actomyosin bundles in spermathecal cells. Confocal maximum intensity projections of excised spermathecae at different developmental stages expressing GFP-labeled myosin (GFP::NMY-1; green) and stained with phalloidin to label F-actin (red). Yellow indicates actin and myosin colocalization. (A) Late L4 animal, (B) young adult prior to the first ovulation, and (C) mature adult after the first ovulation. The spermatheca in C is unoccupied (after oocyte exit). White boxes indicate section magnified in insets below. All insets are a single confocal z-slice showing only the most basal surface. (A’, C’) Actin, (A’’, C’’) myosin, (A’’’, C’’’) merge. Scale bar: 20 μm (A–C), 10 μm (insets).